Miosotis Familia, 48, was shot in the head shortly after midnight while she and her partner were in a marked police truck. She was taken to St. Barnabas Hospital, where she died, the New York Police Department said.
Familia was "assassinated in an unprovoked attack on cops" when a suspect shot through the window of the officer's vehicle, said James O'Neill, the New York police commissioner.
Police encountered a male suspect, Alexander Bonds, 34, running about one block from the scene. The suspect drew a gun as officers confronted him, and he was shot and killed by police, O'Neill said.
A silver revolver was recovered at the scene, police said. The gun was stolen more than four years ago in Charleston, West Virginia, an NYPD source familiar with the investigation told CNN. The gun had two used cartridges in it and two live rounds, the source said.
Dispatch audio of the moments after Familia was shot reveal her partner frantically calling for help.
"Shots fired!" the officer's partner says. "Give me a f****** bus!"
Familia worked in the 46th Precinct, which tweeted out a photo of the officer.
An apparent bystander also was struck by a bullet during the encounter, police said. The person, who was not identified, is in stable condition at a hospital.
The suspect, also known as John Bonds, was arrested in Syracuse in July 2005 for robbery in the first degree, the Onondaga County district attorney said.
Bonds received an eight-year sentence, with five years of post-release supervision, and was transferred to Elmira state prison in 2006, according to a statement from the district attorney.
He also had a criminal record for sale of a controlled substance in New York state, Thomas Mailey, spokesman for the New York Department of Corrections and Community Supervision, said.
He went to prison in March 2004 for illegal drug sales, but was out by January 2005, when he was arrested in the Bronx for entering the subway without paying a fare, Bronx District attorney spokeswoman Patrice O'Shaughnessy said.
'Pain and heartbreak'
Familia had three children, the New York City Patrolmen's Benevolent Association said.
"Fully knowing the dangers that she faced, she suited up in uniform every day and stood tall against those who threaten and terrorize the good folks of the Bronx," the union said in a statement.
A friend, Isabel Roman, said Familia had 12-year-old twins and a 20-year-old child.
"She was a very jovial, happy person," Roman said.
Carlos Corporan, Familia's brother-in-law, said she was an excellent woman and the family was "completely devastated."
In a statement, Attorney General Jeff Sessions said Familia was "murdered in a cowardly, unprovoked attack," and he alluded to other recent ambush-style attacks on police such as those in Dallas
"This murder in cold blood is a tragedy, and sadly it is the latest in a troubling series of attacks on police officers over the past two years," Sessions said.
New York Mayor Bill de Blasio praised Familia and the actions of officers who responded to the shooting.
"She was on duty, serving this city, protecting people, doing what she believed in and doing the job she loved," de Blasio told reporters. "After this sudden and shocking attack, her fellow officers came to her aid immediately."
US Sen. Chuck Schumer called the shooting "terrible news
," and Gov. Andrew Cuomo said all New Yorkers "share in the pain and heartbreak caused by her death."